Reference plate for checking photometric accuracy

With the reference plate 666R013 from Hellma Analytics the photometric accuracy of microplate readers can be checked.

The reference plate has the same dimensions as a microplate with 96 wells and a 6.6 mm diameter per window (H 13.0 x D 127 x L 85.5 mm).

For the five inserted neutral density glasses (columns 3-12) the absorbance value can be measured for 16 windows. Another 16 windows are without glass (columns 1+2) and are used as reference.


The reference plate consists of five neutral density glasses with different nominal absorbance values. Therefore the linearity of the absorbance scale can be checked by plotting the absorbance values for each wavelength in a diagram against the measuring values on the calibration certificate.


Article-No. 666R013  Request this article
Application Reference plate for microplate readers
for checking the photometric accuracy
Content Column 1+2: empty - reference
Column 3+4: ND with 0.25 Abs
Column 5+6: ND with 0.5 Abs
Column 7+8: ND with 1.0 Abs
Column +10: ND with 1.5 Abs
Column 11+12: ND with 2.5 Abs
Standard Certification Photometric accuracy certified with DAkkS calibration certificate at
Wavelengths: 405, 450, 490, 650 nm; at 8 Points in a row
Slit width: 1 nm
Possible Certification Wavelengths: all possible between 405 bis 800 nm;
Slit widths: all up to 2 nm possible


Helpful tool for monitoring mean and target values

Control charts, such as e.g. target value charts are used to graphically display measured values. Here, the value specified in the Hellma Analytics calibration certificate is used as the target value. All measured values must be within the range of the measurement uncertainty, otherwise there is an out-of-control situation.

Photometric Accuracy F2, F3, F4 (Control Charts EN)


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Optical Reference Materials (Brochure & User Manual EN)



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