Chemistry | Biotechnology | Batch-Fermentation

In-line analysis of glucose and ethanol during fermentation with yeast

The automated process control in the chemical and biotechnological industry requires measurement techniques that record the educt and product concentrations in real time either via inline or online. It can also be easily integrated into the process engineering environment. Of the various optical spectroscopy techniques, MIR and Raman spectroscopy have the highest selectivity, since they provide information on the fundamental vibrations of molecules. During the aerobic batch fermentation of Saccharomyces cerevisiae, the degradation of glucose is analyzed and the ethanol concentration is measured.

Product advantages for this application

The major advantage using optical probes is that the measurement technique can be spatially separated from the process allowing additional quantification through an at-line measurement of weighed substance concentrations and fermentation samples. Raman spectroscopy is more selective than NIR and UV / VIS spectroscopy and thus better suited for this application. Furthermore, it is predestined for bioprocess tracking, since the water spectrum has a lower dominance resulting in less interference.


Special features

  • Simple process connection due to a large flange selection
  • Adaptation to other devices and lasers is possible
  • Good process capability through high-quality Kalrez O-rings


Hellma USA Inc.
80 Skyline Drive
11803 Plainview, NY
United States

Phone: +1 5169 3908 88


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