Biotechnology | Basic research | Measurement of protein concentrations

The determination of protein concentrations using UV/Vis spectroscopy is a central component of biochemical analyses

Proteins are of fundamental importance for numerous biological and biochemical processes. In research, precise qualification of proteins enables experiments to be carried out reliably, results to be better compared with each other, and reproducible data to be generated. In biotechnological production, too, the exact determination of protein concentration is important in order to be able to optimize manufacturing processes in a targeted manner. UV/Vis spectroscopy is an established and frequently used method of biochemical analysis for determining proteins in liquid samples both quantitatively and qualitatively.

As part of basic research on cryptochromes and photolysis, UV/Vis spectra of liquid protein samples are recorded in order to obtain information on optimal sample preparation for further analysis. The concentration and condition of the sample are particularly relevant in this context. The measurement serves both quality assurance and standardization purposes, as well as ensuring the comparability of further analyses.

A more detailed description on how this application was realized using online measurement technology can be found in our detailed application report, which you can download from the info column at the top of the page.