Reliable and Efficient Qualification of Sensitive Samples with Low Volumes

Hellma supports basic research on proteins

UV/Vis spectroscopy is an established and routinely used method in biochemical analytics for the quantification and qualification of proteins in liquid samples. By analyzing UV/Vis spectra, important conclusions can be drawn for biotechnological research and production regarding the function and condition of proteins.

In fundamental research on cryptochromes and photolyases, a research group at the Institute of Physical Chemistry at the University of Freiburg measures UV/Vis spectra of liquid protein samples. The aim is to obtain information on optimal sample preparation for further analyses. Of particular relevance are the concentration and the condition of the sample.

Conventional measurements using standard cuvettes are problematic and risky

However, these valuable samples are often available only at low concentrations and in limited volumes. Measurements using standard cuvettes with a fixed path length require dilution followed by recovery of the sample, which is time-consuming and involves significant risks to sample integrity.

Microvolume analysis with the TrayCell 2.0 enalbes dilution free analysis in a standard spectrophotometer

By using our TrayCell 2.0 microvolume measurement cell, these issues are avoided. It allows direct measurement without prior dilution in a standard spectrophotometer. Only minimal sample volumes of approximately 0.7 µl to 5 µl are required.

The consistently precise positioning of the aperture in the measurement beam prevents deviations relative to the reference measurement. In addition, the special design of the TrayCell 2.0 ensures that the sample is not exposed to harmful light influences during measurement.

The advantages of this solution at a glance

• No dilution of the sample required

• Sample integrity guaranteed

• Easy cleaning without removing the cuvette

• Suitable for all common spectrophotometers with a standard cuvette holder

Application Note

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